Serum exosome precipitation is employed as a separation method, which is cost-effective, time-saving, and easy to perform, enabling the entire process—from blood collection to exosome isolation—to be completed within two hours.

Product Model:

DL-Exo-S1, DL-Exo-S2

Product Specifications

6 ml, 12 ml

Storage temperature

+4°C

Introduction to Serum Exosome Isolation Solution

Exosomes are vesicles with a lipid bilayer membrane. Originating from intracellular endocytic compartments, they typically measure 30 to 150 nanometers in diameter and carry bioactive molecules—such as nucleic acids, lipids, certain enzymes, proteins, polysaccharides, and cellular metabolites—from their parent cells. Virtually all cell types, including malignant tumor cells, secrete exosomes. These vesicles are found in various biological fluids, including blood, saliva, urine, and cerebrospinal fluid. By transporting diverse biomolecules—including nucleic acids, proteins, lipids, and metabolites—between cells, exosomes play a critical role in intercellular communication, participating in processes such as immune system regulation, tissue repair, and genetic information transfer. Moreover, exosomes harbor biomarkers specific to their parent cells, making them highly valuable for disease diagnosis, particularly in the context of malignancies. Consequently, there is a strong demand for rapid, stable, and reproducible methods for exosome isolation. Ultracentrifugation is a widely used technique, but it requires expensive equipment. Size-exclusion chromatography (SEC), which separates exosomes based on size, is time-consuming and often fractionates the sample into multiple components. A newer generation of SEC employs the intelligently designed Smart SEC isolation system, combining size exclusion with affinity-based separation; however, this approach remains costly. To address these challenges, Zhuhai Deer Bioengineering Co., Ltd. has introduced DL-EXO-S serum exosome isolation solution, which utilizes a serum‑based exosome precipitation method. This approach is economical, saves time, and is easy to operate, enabling the entire process—from blood collection to exosome extraction—to be completed within two hours.

Product Performance Analysis

1. Compared with commercially available, well-known exosome isolation reagents, the DL-EXO-S serum exosome isolation solution yields a comparable amount of exosomal protein; both reagents can recover more than 1 mg of exosomal protein from 30 µL of serum.

2. Comparison of CD9 expression levels in exosome samples isolated using DL-EXO-S serum exosome isolation reagent; see Figure 1.

Figure 1, Western blot: EXO‑Q represents exosome protein samples isolated using a commercially available exosome isolation reagent, while DL‑EXO‑S corresponds to exosome protein samples isolated with Diel Biotech’s serum‑derived exosome isolation reagent. Each lane was loaded with 25 µg of exosome protein. The primary antibody was a rabbit polyclonal anti‑CD9 antibody, and the secondary antibody was a goat anti‑rabbit immunoglobulin polyclonal antibody conjugated with the near‑infrared fluorescent dye 800CW. Figure 1 shows that the CD9 band in the EXO‑S lane is slightly darker than that in the EXO‑Q lane, suggesting that the exosomes isolated with EXO‑S exhibit higher purity compared to those isolated with EXO‑Q.

3. The particle size and distribution of exosomes isolated from the DL-EXO-S serum‑derived exosome isolation solution were measured using a Zetasizer, and the results were compared with those obtained from exosomes isolated using commercially available exosome isolation kits.

1) Exosomes isolated using the DL-EXO-S serum exosome isolation reagent, as analyzed by Zetasizer, exhibit a particle size distribution predominantly between 20 and 80 nm, with an average size of approximately 35 nm (see Figure 2).

2) The exosomes isolated using the commercial exosome kit are predominantly distributed within the 12–30 nm size range. See Figure 3.